Application of the intracellular gamma interferon assay to recalculate the potency of CD8(+) T-cell responses to herpes simplex virus.
نویسندگان
چکیده
Enumeration and characterization of herpes simplex virus (HSV)-specific CD8(+) T lymphocytes are tedious and indirect. We quantitated antigen-specific CD8(+) T cells during acute and secondary stages of HSV infection using intracellular gamma interferon production upon stimulation with virus or immunodominant peptide. Results show a substantial increase in the number of CD8(+) T cells which was otherwise underestimated with the conventional limiting dilution analysis.
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عنوان ژورنال:
- Journal of virology
دوره 74 12 شماره
صفحات -
تاریخ انتشار 2000